From 97c793912ad11419e742d0c00408355b8132f341 Mon Sep 17 00:00:00 2001 From: Reynaldo Morillo Date: Tue, 19 Dec 2017 08:04:12 -0500 Subject: [PATCH] Just put everything together for the appendum. The README should explain mostly everything that it needs. All the data and scripts have been modified to use relative paths, rather than absolute. Using best configuration of the GAN. Created a requirement.txt for python dependency installing. --- README.md | 57 +++++ gan/__pycache__/gan.cpython-35.pyc | Bin 0 -> 1602 bytes gan/gan.py | 213 ++++++++++-------- gan/new_samples.csv | 35 +++ gan/training_record.csv | 3 + .../class_dataset/human_and_mouse_dataset.csv | 187 +++++++++++++++ .../human_and_mouse_dataset_log2_ratio.csv | 49 ++++ .../datasets/class_dataset/human_dataset.csv | 21 ++ .../human_dataset_log2_ratio.csv | 11 + .../class_dataset/human_mouse_dataset.csv | 187 +++++++++++++++ .../datasets/class_dataset/mouse_dataset.csv | 167 ++++++++++++++ .../mouse_dataset_log2_ratio.csv | 43 ++++ mkdataset/distribution_analysis.R | 14 +- mkdataset/get_gene_intersection_list.r | 2 +- mkdataset/get_gse_data.r | 21 +- mkdataset/mkdataset.py | 37 --- mkdataset/mkdataset.r | 16 +- mkdataset/mkdataset_log2_columns.r | 16 +- mkdataset/prep_thams_data.r | 6 +- requirements.txt | 62 +++++ 20 files changed, 972 insertions(+), 175 deletions(-) create mode 100644 README.md create mode 100644 gan/__pycache__/gan.cpython-35.pyc create mode 100644 gan/new_samples.csv create mode 100644 gan/training_record.csv create mode 100644 mkdataset/datasets/class_dataset/human_and_mouse_dataset.csv create mode 100644 mkdataset/datasets/class_dataset/human_and_mouse_dataset_log2_ratio.csv create mode 100644 mkdataset/datasets/class_dataset/human_dataset.csv create mode 100644 mkdataset/datasets/class_dataset/human_dataset_log2_ratio.csv create mode 100644 mkdataset/datasets/class_dataset/human_mouse_dataset.csv create mode 100644 mkdataset/datasets/class_dataset/mouse_dataset.csv create mode 100644 mkdataset/datasets/class_dataset/mouse_dataset_log2_ratio.csv delete mode 100644 mkdataset/mkdataset.py create mode 100644 requirements.txt diff --git a/README.md b/README.md new file mode 100644 index 0000000..268c634 --- /dev/null +++ b/README.md @@ -0,0 +1,57 @@ +Data Wrangling & GAN +================================= + +## R Dependencies + +When using R I like using [RStudio](https://www.rstudio.com). I think it's the best IDE for R, and makes iterating on code very easy and quick. Within RStudio there is a package manager that can help you install the packages I have listed here: + +* dyplr +* ggplot2 + +The following two packages are installed a little differently. +First install [Bioconductor](http://bioconductor.org/) +Then you may install [GEOQuery](http://genomicsclass.github.io/book/pages/GEOquery.html) + +Those two packages are used in [get_gse_data.r](./mkdataset/get_gse_data.r), which can get any GSE, given the GSE ID and gene symbol column name. + + +## Python Dependencies + +I used a virtual environment by [virtualenv](https://pypi.python.org/pypi/virtualenv). If you want to use it as well, I recommend [this installation tutorial](http://docs.python-guide.org/en/latest/dev/virtualenvs/). + +> **NOTE**: Using a virtual environment doesn't allows you to use [matplotlib](http://matplotlib.org/faq/virtualenv_faq.html) directly. You need to map it to your system's copy of **matplotlib** because the graphics libraries to create window frames is closely tied to the operating system. + +The prominent packages are: + +* numpy +* Pandas +* Scikit-Learn +* TensorFlow +* Keras +* keras_adversarial + +To install all the dependencies quickly and easily you should use [`pip`](https://pypi.python.org/pypi/pip/) + +```bash +pip install -r requirements.txt +``` + +## How to Run any Script + +Just navigate to the folder containing the script, and run it directly. + +### R + +If you're using RStudio, then all you need to do is **source** the script. There is a button for that in the top right corner of the editor window. Else from the command line: + +```r +R CMD BATCH +``` + +### Python + +Just run it directly from the command line. Assuming that you environment is prepared, and you have all the dependencies, all you have to do is + +``` +python gan.py +``` diff --git a/gan/__pycache__/gan.cpython-35.pyc b/gan/__pycache__/gan.cpython-35.pyc new file mode 100644 index 0000000000000000000000000000000000000000..10811f4431f7b5130949abb4d64c061a10782b7e GIT binary patch literal 1602 zcmZWp&2QX96o2;G`@P8~ZMNAYO$-ff7l~b{gt%0QiWL`#jgPg%DoRf1W^Z42hZ8mfHy!ifi$ag0K5gF4WwP2L*N|{T_9Z$ zJs>?0Ye3dOtOHpG(FfA6^yE4eiy_Rkge+61U>+956Hdh+ycH0 zVhCgiVh6|$h+QDNm8@OL_JHhxxDVt$ayyW8;TNoN9}-OF01`|l0v5pX17HBl4`KNL z*a5Hzu6(#c1P*~6!tx^r4!*x4AnP}qV;_d(A+UX54@P=N(YKnWGPcav*knAl$ZKk5sK_|OeVx;Drp+iU!f$1k+ajDYX;{&iYLzKG zw@dbSPOE7Az|4YXY~&a1Blb3D(x#Lb;R}^8j?RK-2^IKle6Q8~4b`Js(SMelqJOGU zu~aMCCUvpnIh92RNo=W}Gi%UkbW8bFW>b+~FnyGYoLefH%1tqlN(;(kPAvwH%Q8|q zpZTs{d;(kzTwmidI9zavAR{mU?|pdpBUrEGAgUe=5w7XaQ+QtkTPxW(aVYT}>X$We zc;mQTVk1xxBD(&i56k~4J$)O&Ym^*~>KCuu5kAk;Qzj!CJ^5rBWmG2AL+N`ZUfqj4y^H6I2jlTXoZKWjx%nFBXO+E~ 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pd.read_csv(os.path.join(base_path, file_name)) # Prepare Data #--------------------------------------- -just_values = all_data.iloc[:, 3:] -new_sample = permute_sample(just_values, 5) -real_dataset = make_3d_dataset(just_values, 5, 2) -print(real_dataset.shape) -print(real_dataset) - -# # Prepare data -# x_train = all_data.iloc[:, np.arange(20)] -# -# # column_start_index_of_genes = 2 -# # class_label_column_index = 1 -# # features = all_data.iloc[:, np.arange(column_start_index_of_genes, df.shape[1])] -# # labels = all_data.iloc[:, class_label_column_index] -# -# # column_start_index_of_genes = 2 -# # features = all_data.iloc[:, np.arange(20)] -# # labels = labeled_data.iloc[:, class_label_column_index] -# -# # x_train, x_test, y_train, y_test = train_test_split(features, labels, test_size=0.33, random_state=42) -# -# -# ################################################# -# # Variables -# ################################################# -# -# # Output -# output_base_path = './' -# -# #--------------------------------------- -# # Network Variables -# #--------------------------------------- -# -# input_dimension = x_train.shape[1] # Number of features (e.g. genes) -# -# gen_input_shape = (input_dimension,) -# discr_input_shape = (input_dimension,) -# -# epochs = 10 -# batch_size = x_train.shape[0] -# -# # Build Generative model -# generative_model = Sequential() -# # generative_model.add(InputLayer(input_shape=gen_input_shape)) -# generative_model.add(Dense(units=int(1.2*input_dimension), activation='relu', input_dim=input_dimension)) -# generative_model.add(Dropout(rate=0.2, noise_shape=None, seed=15)) -# generative_model.add(Dense(units=int(0.2*input_dimension), activation='relu')) -# generative_model.add(Dense(units=input_dimension, activation='relu')) -# generative_model.add(Reshape(discr_input_shape)) -# -# # Build Discriminator model -# discriminator_model = Sequential() -# discriminator_model.add(InputLayer(input_shape=discr_input_shape)) -# discriminator_model.add(Dense(units=int(1.2*input_dimension), activation='relu')) -# discriminator_model.add(Dropout(rate=0.2, noise_shape=None, seed=75)) -# discriminator_model.add(Dense(units=int(0.2*input_dimension), activation='relu')) -# discriminator_model.add(Dense(units=1, activation='sigmoid')) -# -# # Build GAN -# gan = simple_gan(generative_model, discriminator_model, normal_latent_sampling((input_dimension, ))) -# model = AdversarialModel(base_model=gan, -# player_params=[generative_model.trainable_weights, -# discriminator_model.trainable_weights], -# player_names=['generator', 'discriminator']) -# # Other optimizer to try AdversarialOptimizerAlternating -# model.adversarial_compile(adversarial_optimizer=AdversarialOptimizerSimultaneous(), -# player_optimizers=['adam', 'adam'], loss='binary_crossentropy') -# -# # Print Summary of Models -# generative_model.summary() -# discriminator_model.summary() -# gan.summary() -# -# # Train -# # gan_targets takes as inputs the # of samples -# training_record = model.fit(x=x_train, y=gan_targets(x_train.shape[0]), epochs=epochs, -# batch_size=batch_size) -# -# # Diplay plot of loss over training -# # plt.plot(history.history['player_0_loss']) -# # plt.plot(history.history['player_1_loss']) -# # plt.plot(history.history['loss']) -# -# # Predict (i.e. produce new samples) -# zsamples = np.random.normal(size=(1, input_dimension)) -# pred = generative_model.predict(zsamples) -# print(pred) -# -# # Save new samples to file -# # new_samples = pd.DataFrame(pred) -# # new_samples.to_csv(os.path.join(output_base_path, 'new_samples.csv')) -# -# # # save training_record -# # df = pd.DataFrame(training_record.history) -# # df.to_csv(os.path.join(output_base_path, 'training_record.csv')) -# # -# # # save models -# # generator.save(os.path.join(output_base_path, 'generator.h5')) -# # discriminator.save(os.path.join(output_base_path, "discriminator.h5")) +just_values = all_data.iloc[1:, 3:] +x_train = make_3d_dataset(just_values, 300, 100) + +################################################# +# Variables +################################################# + +# Output +output_base_path = './' + +#--------------------------------------- +# Network Variables +#--------------------------------------- + +# Layer Input Output Shapes +gen_input_shape = (x_train.shape[1], x_train.shape[2]) +discr_input_shape = (x_train.shape[1], x_train.shape[2]) + +gen_output_shape = discr_input_shape + +# Generator Variables +weight_initializer = initializers.RandomNormal(mean=0.0, stddev=0.05, seed=None) +bias_initializer = initializers.RandomUniform(minval=-0.05, maxval=0.05, seed=None) +generator_regularizer = regularizers.l1(0.000000005) +descriminator_regularizer = regularizers.l2(0.0000000005) + +# Training Varaibles +epochs = 2 +batch_size = 1 # x_train.shape[0] +input_size = x_train.shape[1] * x_train.shape[2] * batch_size + +# Build Generative model +generative_model = Sequential() +# generative_model.add(InputLayer(input_shape=gen_input_shape)) +generative_model.add(InputLayer(input_shape=discr_input_shape)) +generative_model.add(Flatten()) +generative_model.add(Dense(units=int(1.5*input_size), + use_bias=True, + activation='relu', + kernel_initializer=weight_initializer, + bias_initializer=bias_initializer, + kernel_regularizer=generator_regularizer)) +# generative_model.add(ActivityRegularization(l1=0.02)) +generative_model.add(Dense(units=int(input_size), + activation='relu', + kernel_initializer=weight_initializer, + kernel_regularizer=generator_regularizer)) +generative_model.add(Reshape(discr_input_shape)) + +# Build Discriminator model +discriminator_model = Sequential() +discriminator_model.add(InputLayer(input_shape=discr_input_shape)) +discriminator_model.add(BatchNormalization(axis=1)) +discriminator_model.add(Flatten()) +discriminator_model.add(Dense(units=int(1.5*input_size), + use_bias=True, + activation='relu', + kernel_initializer=weight_initializer, + bias_initializer=bias_initializer, + kernel_regularizer=descriminator_regularizer)) +# discriminator_model.add(ActivityRegularization(l2=0.02)) +discriminator_model.add(Dense(units=int(0.2*input_size), + activation='relu', + kernel_initializer=weight_initializer, + bias_initializer=bias_initializer, + kernel_regularizer=descriminator_regularizer)) +discriminator_model.add(Dense(units=1, activation='sigmoid')) + +# Build GAN +gan = simple_gan(generative_model, discriminator_model, normal_latent_sampling(gen_input_shape)) +model = AdversarialModel(base_model=gan, + player_params=[generative_model.trainable_weights, + discriminator_model.trainable_weights], + player_names=['generator', 'discriminator']) +# Other optimizer to try AdversarialOptimizerAlternating +model.adversarial_compile(adversarial_optimizer=AdversarialOptimizerSimultaneous(), + player_optimizers=['adam', 'adam'], loss='binary_crossentropy') + +# Print Summary of Models +generative_model.summary() +discriminator_model.summary() +gan.summary() + +# Train +# gan_targets takes as inputs the # of samples +training_record = model.fit(x=x_train, + y=gan_targets(x_train.shape[0]), + epochs=epochs, + batch_size=batch_size) + +# Diplay plot of loss over training +# plt.plot(history.history['player_0_loss']) +# plt.plot(history.history['player_1_loss']) +# plt.plot(history.history['loss']) + +# Predict (i.e. produce new samples) +zsamples = np.random.normal(size=(1, x_train.shape[1], x_train.shape[2])) +pred = generative_model.predict(zsamples) +print(pred) + +# Save new samples to file +new_samples = pd.DataFrame(pred[0,:,:]) +new_samples.to_csv(os.path.join(output_base_path, 'new_samples.csv')) + +# save training_record +df = pd.DataFrame(training_record.history) +df.to_csv(os.path.join(output_base_path, 'training_record.csv')) + +# save models +generative_model.save(os.path.join(output_base_path, 'generator.h5')) 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diff --git a/gan/training_record.csv b/gan/training_record.csv new file mode 100644 index 0000000..6aa3293 --- /dev/null +++ b/gan/training_record.csv @@ -0,0 +1,3 @@ +,discriminator_loss,discriminator_yfake_loss,discriminator_yreal_loss,generator_loss,generator_yfake_loss,generator_yreal_loss,loss +0,0.09893678217194975,0.045659461609743775,0.03306951967803035,31.80522808790207,16.014377937316898,15.770637784896378,31.904164775212607 +1,0.013621237700184186,1.0000001537946446e-07,1.192093321833454e-07,32.07410971323649,16.11809539794922,15.942384719848633,32.087730929056804 diff --git a/mkdataset/datasets/class_dataset/human_and_mouse_dataset.csv b/mkdataset/datasets/class_dataset/human_and_mouse_dataset.csv new file mode 100644 index 0000000..ff5ec86 --- /dev/null +++ b/mkdataset/datasets/class_dataset/human_and_mouse_dataset.csv @@ -0,0 +1,187 @@ +"gsm" "species" "study_type" "acta2" "alb" "ambp" "apoa2" "col12a1" "dnase1l3" "fhl2" "gc" "hpgd" "lmcd1" "lox" "ltbp2" "mrc1" "nt5dc2" 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b/mkdataset/datasets/class_dataset/human_and_mouse_dataset_log2_ratio.csv new file mode 100644 index 0000000..422d925 --- /dev/null +++ b/mkdataset/datasets/class_dataset/human_and_mouse_dataset_log2_ratio.csv @@ -0,0 +1,49 @@ 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diff --git a/mkdataset/datasets/class_dataset/human_dataset.csv b/mkdataset/datasets/class_dataset/human_dataset.csv new file mode 100644 index 0000000..da7af7b --- /dev/null +++ b/mkdataset/datasets/class_dataset/human_dataset.csv @@ -0,0 +1,21 @@ 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diff --git a/mkdataset/datasets/class_dataset/human_dataset_log2_ratio.csv b/mkdataset/datasets/class_dataset/human_dataset_log2_ratio.csv new file mode 100644 index 0000000..5947164 --- /dev/null +++ b/mkdataset/datasets/class_dataset/human_dataset_log2_ratio.csv @@ -0,0 +1,11 @@ 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diff --git a/mkdataset/datasets/class_dataset/human_mouse_dataset.csv b/mkdataset/datasets/class_dataset/human_mouse_dataset.csv new file mode 100644 index 0000000..8969a64 --- /dev/null +++ b/mkdataset/datasets/class_dataset/human_mouse_dataset.csv @@ -0,0 +1,187 @@ 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diff --git a/mkdataset/datasets/class_dataset/mouse_dataset.csv b/mkdataset/datasets/class_dataset/mouse_dataset.csv new file mode 100644 index 0000000..b7ba209 --- /dev/null +++ b/mkdataset/datasets/class_dataset/mouse_dataset.csv @@ -0,0 +1,167 @@ +gsm,species,study_type,acta2,aebp1,ahnak2,alb,ambp,apoa2,cdh3,ces3a,ch25h,chst11,clec4g,col12a1,cpxm1,cyp2f2,d0h4s114,dnase1l3,ereg,fam71a,fgl2,fhl2,gc,gm2083,gpihbp1,gprc5b,hpgd,lmcd1,loc100861909,loc100862155,lox,ltbp2,mrc1,mup1,mup10,mup11,mup12,mup13,mup14,mup15,mup19,mup2,mup3,mup7,mup8,nrxn2,nt5dc2,pdpn,pi15,pla1a,plat,prrx1,ptgs2,rasl11b,rgs16,rgs4,rnu3a,serpina1c,serpine2,slit3,stab2,svep1,sytl2,tgfb3,thbs1,timp1,tnc,tnfaip6,vcan,vipr1,vsig4 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a/mkdataset/datasets/class_dataset/mouse_dataset_log2_ratio.csv b/mkdataset/datasets/class_dataset/mouse_dataset_log2_ratio.csv new file mode 100644 index 0000000..cdda303 --- /dev/null +++ b/mkdataset/datasets/class_dataset/mouse_dataset_log2_ratio.csv @@ -0,0 +1,43 @@ 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diff --git a/mkdataset/distribution_analysis.R b/mkdataset/distribution_analysis.R index 396154f..6fd189c 100644 --- a/mkdataset/distribution_analysis.R +++ b/mkdataset/distribution_analysis.R @@ -4,7 +4,7 @@ library(ggplot2) # Load Data ############################# -base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' +base_path <- './' file_name <- 'datasets/gan_datasets/tham_lasso_dataset.csv' all_data <- read.csv(paste(base_path, file_name, sep=''), header=TRUE) @@ -18,7 +18,7 @@ gene_list <- read.csv(paste(base_path, gene_list_file_name, sep=''), header=TRUE ################################ # Function from https://stackoverflow.com/questions/22742737/function-to-save-ggplot -# Special Thanks to enricoferrero +# Special Thanks to enricoferrero # https://stackoverflow.com/users/1540663/enricoferrero savePlot <- function(myPlot, file_name) { png(paste(file_name, '.png', sep=''), width=732, height=543) @@ -27,7 +27,7 @@ savePlot <- function(myPlot, file_name) { } ################################ -# Variables +# Variables ################################ # Vector of fields to examine @@ -39,16 +39,14 @@ for (field in gene_list$Symbol) { if (field %in% colnames(all_data)) { the_title <- paste('Expression Historgram of', field) x_lab <- paste(field, 'Expression Value') - the_plot <- ggplot(all_data, aes(x=all_data[ , field])) + - geom_histogram() + + the_plot <- ggplot(all_data, aes(x=all_data[ , field])) + + geom_histogram() + geom_vline(xintercept=mean(all_data[ , field]), color='red', size=2) + labs(title=the_title, x=x_lab) print(the_plot) savePlot(the_plot, field) - + # Pause until ready for next readline("Press Enter for next") } } - - diff --git a/mkdataset/get_gene_intersection_list.r b/mkdataset/get_gene_intersection_list.r index ef3528c..1004a9e 100644 --- a/mkdataset/get_gene_intersection_list.r +++ b/mkdataset/get_gene_intersection_list.r @@ -1,7 +1,7 @@ get_gene_list <- function(gene_file_name) { - base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' + base_path <- './' genes_list_file_path <- paste(base_path, 'human_mouse_gene_lists/', gene_file_name, sep='') genes_list_data <- read.table(genes_list_file_path, sep='\t', header=TRUE) genes_list_data$Symbol <- tolower(genes_list_data$Symbol) diff --git a/mkdataset/get_gse_data.r b/mkdataset/get_gse_data.r index 7802602..6dc2590 100644 --- a/mkdataset/get_gse_data.r +++ b/mkdataset/get_gse_data.r @@ -1,31 +1,32 @@ library(GEOquery) # Set libcurl as the prefered method to use when downloading files -options(download.file.method.GEOquery='libcurl') +options(download.file.method.GEOquery='libcurl') # Create function to get expression set of given gse # along with the gene symbols -get_gse_expression_set <- function(gse_name) { +get_gse_expression_set <- function(gse_name, symbol_col_name) { # Get the GSE by name and create a dataframe out of the expression set gse <- getGEO(gse_name, GSEMatrix = TRUE)[[1]] gse_df <- as.data.frame(exprs(gse)) gse_df$probe_id <- rownames(gse_df) - + # Extract Gene Symbols and associated Probe IDs feature_data <- attr(gse, "featureData") - symbols <- attr(feature_data, "data")$Symbol + symbols <- attr(feature_data, "data")[ , symbol_col_name] probe_ids <- attr(feature_data, "data")$Probe_Id - + # Create a dataframe to hold the symbols and probe IDs symbol_df <- data.frame(symbol=numeric(length(symbols)), probe_id=character(length(probe_ids)) ) symbol_df$symbol <- symbols symbol_df$probe_id <- probe_ids - - # Merge the dataframes together + + # Merge the dataframes together complete_data <- merge(x=gse_df, y=symbol_df, by='probe_id', all.x = TRUE) } -base_path = "./" # where to put the resulting file -gse_name = 'GSE68000' -my_data <- get_gse_expression_set(gse_name) +base_path <- "./" # where to put the resulting file +gse_name <- 'GSE52253' +symbol_col_name <- 'Symbol' +my_data <- get_gse_expression_set(gse_name, 'Symo') write.table(my_data, file=paste(base_path, gse_name, ".csv", sep=""), sep = '\t', row.names=FALSE) diff --git a/mkdataset/mkdataset.py b/mkdataset/mkdataset.py deleted file mode 100644 index fd91b92..0000000 --- a/mkdataset/mkdataset.py +++ /dev/null @@ -1,37 +0,0 @@ -import numpy as np -import pandas as pd -import glob - -genes_list_file_path = './human_mouse_gene_lists/mouse_genes.csv' -genes_list_data = pd.read_csv(genes_list_file_path, sep='\t') -genes_list_data.loc[:, 'Symbol'] = genes_list_data.loc[:, 'Symbol'].str.lower() -# genes_list = list(genes_list_data.loc[:, 'Symbol']) -genes_list = ['ACTAC2'] - -# Read in the files -# Get master metadata file -metadata_file_path = './class_data/metadata/gse_metadata.csv' -metadata = pd.read_csv(metadata_file_path, sep='\t') -metadata = metadata.set_index('gse_id') - -gse_file_path = './class_data/csv/clean_csv/without_log2/' -for gse_file in glob.glob('{0}*.csv'.format(gse_file_path)): - gse_data = pd.read_csv(gse_file, sep='\t') - gse_data.loc[:, 'Symbol'] = gse_data.loc[:, 'Symbol'].str.lower() - gse_data = gse_data.set_index('Symbol') - t_gse_data = gse_data.transpose() - print(t_gse_data.head()) - print(t_gse_data.index) - selected_data = t_gse_data.index.get_loc(genes_list) - print(selected_data) - break # TODO: Remove once done debugging - -# Get Metadata - -# Remove Symbols with no name - -# Make all Symbols lower case - -# Transpose the dataframe - -# Add to database diff --git a/mkdataset/mkdataset.r b/mkdataset/mkdataset.r index 6f83bad..1357c33 100644 --- a/mkdataset/mkdataset.r +++ b/mkdataset/mkdataset.r @@ -2,11 +2,11 @@ library(dplyr) library(data.table) # Constants -base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' +base_path <- './' # Functions get_gene_list <- function(gene_file_name) { - base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' + base_path <- './' genes_list_file_path <- paste(base_path, 'human_mouse_gene_lists/', gene_file_name, sep='') genes_list_data <- read.table(genes_list_file_path, sep='\t', header=TRUE) genes_list_data$Symbol <- tolower(genes_list_data$Symbol) @@ -56,7 +56,7 @@ metadata <- read.table(metadata_file_path, sep='\t', header=TRUE) # Remove Symbols with no name -gse_file_path <- paste(base_path, '/class_data/csv/clean_csv/without_log2/', sep='') +gse_file_path <- paste(base_path, 'class_data/csv/clean_csv/without_log2/', sep='') gse_file_list <- list.files(gse_file_path, pattern="*.csv", full.names=TRUE, recursive=FALSE) all_genes <- data.frame(Symbol=genes_list) for (gse_file in gse_file_list) { @@ -64,20 +64,20 @@ for (gse_file in gse_file_list) { gse_name = strsplit(strsplit(gse_file, "[/]")[[1]][length(strsplit(gse_file, "[/]")[[1]])], "[.]")[[1]][1] species <- metadata[metadata$gse_id == gse_name, 'species'] study_type <- metadata[metadata$gse_id == gse_name, 'study_type'] - + if (species %in% selected_species) { gse_data <- read.table(gse_file, sep='\t', header=TRUE) - gse_data <- gse_data[ , c(-2, -3)] # Remove the probe ID columns + gse_data <- gse_data[ , c(-2, -3)] # Remove the probe ID columns # Convert the symbols to all lowercase gse_data$Symbol <- tolower(gse_data$Symbol) selected_genes <- as.data.frame(filter(gse_data, Symbol %in% genes_list)) - + if (length(selected_genes$Symbol) > 0) { # Average selected genes non_symbol_columns <- colnames(selected_genes)[-1] selected_avg_genes <- aggregate(selected_genes[ , non_symbol_columns], by=list(selected_genes$Symbol), data=selected_genes, FUN = mean) - + species_df <- as.data.frame(t(data.frame(species=c('species', as.character(rep(species, length(colnames(selected_avg_genes))-1)))))) study_type_df <- as.data.frame(t(data.frame(study_type=c('study_type', as.character(rep(study_type, length(colnames(selected_avg_genes))-1)))))) names(species_df) <- names(selected_avg_genes) @@ -101,4 +101,4 @@ t_all_genes <- make_numeric(t_all_genes, 4:length(colnames(t_all_genes))) t_all_genes_log_2 <- log_columns(t_all_genes, 4:length(colnames(t_all_genes))) output_file_path <- paste(base_path, output_file_name, sep='') -write.table(t_all_genes_log_2, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) \ No newline at end of file +write.table(t_all_genes_log_2, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) diff --git a/mkdataset/mkdataset_log2_columns.r b/mkdataset/mkdataset_log2_columns.r index 094d8ad..1853193 100644 --- a/mkdataset/mkdataset_log2_columns.r +++ b/mkdataset/mkdataset_log2_columns.r @@ -2,11 +2,11 @@ library(dplyr) library(data.table) # Constants -base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' +base_path <- './' # Functions get_gene_list <- function(gene_file_name) { - base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' + base_path <- './' genes_list_file_path <- paste(base_path, 'human_mouse_gene_lists/', gene_file_name, sep='') genes_list_data <- read.table(genes_list_file_path, sep='\t', header=TRUE) genes_list_data$Symbol <- tolower(genes_list_data$Symbol) @@ -43,22 +43,22 @@ for (gse_file in gse_file_list) { gse_name = strsplit(strsplit(gse_file, "[/]")[[1]][length(strsplit(gse_file, "[/]")[[1]])], "[.]")[[1]][1] species <- metadata[metadata$gse_id == gse_name, 'species'] study_type <- metadata[metadata$gse_id == gse_name, 'study_type'] - + if (species %in% selected_species) { gse_data <- read.table(gse_file, sep='\t', header=TRUE) - gse_data <- gse_data[ , c(-2, -3)] # Remove the probe ID columns + gse_data <- gse_data[ , c(-2, -3)] # Remove the probe ID columns gse_data <- gse_data[ , grepl('_', colnames(gse_data), fixed=TRUE) | grepl('Symbol', colnames(gse_data), fixed=TRUE)] - colnames(gse_data) <- c('Symbol', paste(gse_name, '_', colnames(gse_data)[-1], sep='')) + colnames(gse_data) <- c('Symbol', paste(gse_name, '_', colnames(gse_data)[-1], sep='')) # Convert the symbols to all lowercase gse_data$Symbol <- tolower(gse_data$Symbol) selected_genes <- as.data.frame(filter(gse_data, Symbol %in% genes_list)) - + if (length(selected_genes$Symbol) > 0) { # Average selected genes non_symbol_columns <- colnames(selected_genes)[-1] selected_avg_genes <- aggregate(selected_genes[ , non_symbol_columns], by=list(selected_genes$Symbol), data=selected_genes, FUN = mean) - + species_df <- as.data.frame(t(data.frame(species=c('species', as.character(rep(species, length(colnames(selected_avg_genes))-1)))))) study_type_df <- as.data.frame(t(data.frame(study_type=c('study_type', as.character(rep(study_type, length(colnames(selected_avg_genes))-1)))))) names(species_df) <- names(selected_avg_genes) @@ -79,4 +79,4 @@ t_all_genes$gsm <- rownames(t_all_genes) t_all_genes <- t_all_genes[ ,c('gsm','species', 'study_type', colnames(t_all_genes)[!(colnames(t_all_genes) %in% c('gsm','species', 'study_type'))])] output_file_path <- paste(base_path, output_file_name, sep='') -write.table(t_all_genes, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) \ No newline at end of file +write.table(t_all_genes, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) diff --git a/mkdataset/prep_thams_data.r b/mkdataset/prep_thams_data.r index 4fe5d2b..4f87826 100644 --- a/mkdataset/prep_thams_data.r +++ b/mkdataset/prep_thams_data.r @@ -2,11 +2,11 @@ library(dplyr) library(data.table) # Constants -base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' +base_path <- './' # Functions get_gene_list <- function(gene_file_name) { - base_path <- '/home/reynaldo/Documents/School/Fall2017/DataMining/grp_proj/mkdataset/' + base_path <- './' genes_list_file_path <- paste(base_path, 'human_mouse_gene_lists/', gene_file_name, sep='') genes_list_data <- read.table(genes_list_file_path, sep='\t', header=TRUE) genes_list_data$Symbol <- tolower(genes_list_data$Symbol) @@ -44,4 +44,4 @@ final_data_set <- final_data_set[ ,c('test','control', 'class', colnames(final_d final_data_set <- final_data_set[-1, ] output_file_path <- paste(base_path, output_file_name, sep='') -write.table(final_data_set, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) \ No newline at end of file +write.table(final_data_set, file=output_file_path, row.names = FALSE, sep=',', quote=FALSE) diff --git a/requirements.txt b/requirements.txt new file mode 100644 index 0000000..63c2877 --- /dev/null +++ b/requirements.txt @@ -0,0 +1,62 @@ +bleach==1.5.0 +cycler==0.10.0 +decorator==4.1.2 +entrypoints==0.2.3 +enum34==1.1.6 +h5py==2.7.1 +html5lib==0.9999999 +ipykernel==4.7.0 +ipython==6.2.1 +ipython-genutils==0.2.0 +ipywidgets==7.0.5 +jedi==0.11.1 +Jinja2==2.10 +jsonschema==2.6.0 +jupyter==1.0.0 +jupyter-client==5.1.0 +jupyter-console==5.2.0 +jupyter-core==4.4.0 +Keras==2.1.2 +keras-adversarial==0.0.3 +Markdown==2.6.9 +MarkupSafe==1.0 +matplotlib==2.1.0 +mistune==0.8.3 +nbconvert==5.3.1 +nbformat==4.4.0 +notebook==5.2.2 +numpy==1.13.3 +pandas==0.21.0 +pandocfilters==1.4.2 +parso==0.1.1 +pexpect==4.3.1 +pickleshare==0.7.4 +pkg-resources==0.0.0 +prompt-toolkit==1.0.15 +protobuf==3.5.0.post1 +ptyprocess==0.5.2 +Pygments==2.2.0 +pyparsing==2.2.0 +python-dateutil==2.6.1 +pytz==2017.3 +PyYAML==3.12 +pyzmq==16.0.3 +qtconsole==4.3.1 +scikit-learn==0.19.1 +scipy==1.0.0 +simplegeneric==0.8.1 +sip==4.19.6 +six==1.11.0 +tensorflow==1.4.0 +tensorflow-tensorboard==0.4.0rc3 +terminado==0.8.1 +testpath==0.3.1 +Theano==1.0.0 +tk-tools==0.4.2 +tornado==4.5.2 +traitlets==4.3.2 +wcwidth==0.1.7 +Werkzeug==0.12.2 +widgetsnbextension==3.0.8 +xlrd==1.1.0 +xlwt==1.3.0