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# TriPOINT | ||
A pathway analysis tool that integrates non-coding regulators for further gene prioritization. | ||
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Binary file: tripoint.jar | ||
**Binary** file: tripoint.jar | ||
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Available datasets are in the data directory. | ||
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Usage: java -jar tripoint.jar <expression file> -db <pathway database> -o <output directory> | ||
**Usage:** java -jar tripoint.jar <expression file> -db <pathway database> -o <output directory> | ||
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#Parameters | ||
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-db Sets pathway database to import pathways from GRAPHITE. Values available: "kegg", "reactome", "biocarta", "humancyc", "nci", "panther" | ||
**-db** Sets pathway database to import pathways from GRAPHITE. Values available: "kegg", "reactome", "biocarta", "humancyc", "nci", "panther" | ||
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-o Sets output directory for saving result files. REQUIRED | ||
**-o** Sets output directory for saving result files. REQUIRED | ||
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-gid Sets the conversion method for gene identifiers. Refer to 'convertIdentifiers' in GRAPHITE. Default: "SYMBOL" (Currently other identifiers are not recommended) | ||
**-gid** Sets the conversion method for gene identifiers. Refer to 'convertIdentifiers' in GRAPHITE. Default: "SYMBOL" (Currently other identifiers are not recommended) | ||
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-up Sets the up-regulated gene threshold. Default: 0.05 | ||
**-up** Sets the up-regulated gene threshold. Default: 0.05 | ||
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-down Sets the down-regulated gene threshold. Default: -0.05 | ||
**-down** Sets the down-regulated gene threshold. Default: -0.05 | ||
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-w Sets the weak activation/inhibition factor. Default: 0.5 | ||
**-w** Sets the weak activation/inhibition factor. Default: 0.5 | ||
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-p Controls the impact of gene expression when calculating measures. Default: 0.25 | ||
**-p** Controls the impact of gene expression when calculating measures. Default: 0.25 | ||
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-r Controls the rate of exponential decay to control edge distance in impact score calculation. Default: 0.25 | ||
**-r** Controls the rate of exponential decay to control edge distance in impact score calculation. Default: 0.25 | ||
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-perm Sets the number of permutations for p-value calculations. Default: 10000 | ||
**-perm** Sets the number of permutations for p-value calculations. Default: 10000 | ||
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-rstate Sets the random state for reproducible results. Default: Current Time (milliseconds) | ||
**-rstate** Sets the random state for reproducible results. Default: Current Time (milliseconds) | ||
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-refflat Specifies the UCSC refflat file to use to identify TSS locations of genes in pathways. Required for identifying non-coding regulators. Gene identifiers will need to match those provided in the expression file. | ||
**-refflat** Specifies the UCSC refflat file to use to identify TSS locations of genes in pathways. Required for identifying non-coding regulators. Gene identifiers will need to match those provided in the expression file. | ||
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-ci Sets the tab-delimited file for chromatin interaction data to identify non-coding targets of genes | ||
**-ci** Sets the tab-delimited file for chromatin interaction data to identify non-coding targets of genes | ||
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-nct Sets the bed file for non-coding targets to be associated with genes. If chromatin interactions are provided these sites will be connected via chromatin interactions, otherwise it will be proximity based | ||
**-nct** Sets the bed file for non-coding targets to be associated with genes. If chromatin interactions are provided these sites will be connected via chromatin interactions, otherwise it will be proximity based | ||
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-proxSets the proximity based non-coding target threshold which spans the base pair distance both upstream and downstream from the transcription start site. Default: 50000 | ||
**-prox** Sets the proximity based non-coding target threshold which spans the base pair distance both upstream and downstream from the transcription start site. Default: 50000 |